B-909

Liquid Encapsulation Procedure

Section B — Manufacturing Revision 1 7 pages

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1.0 Purpose 
 
 The purpose of this procedure is to outline the process for liquid encapsulation of product
 
 manufactured by Ion Labs, Inc. 
 
 2.0 Scope 
 
 This procedure applies to all liquid products that require encapsulation at lon Labs, Inc.
 
 3.0 Responsibility 
 
 3.1 It is the responsibility of Production (Liquid Encapsulation) Personnel to follow this
 procedure. 
 
 3.2 It is the responsibility of Facility (Cleaning) Personnel to ensure that all holding drums
 and lids are wiped down prior to removing from the encapsulation area.
 
 3.3. ‘It is the responsibility of Production Management to implement this procedure and to
 ensure that all involved personnel have been properly trained to follow this procedure.
 
 3.4 It is the responsibility of QC Personnel to ensure that this procedure is being followed.
 
 4.0 Definitions 
 
 4.1 BPR — Batch Production Record 
 
 4.2 QC- Quality Control 
 
 4.3 PITT — Pallet Inspection Transfer Ticket 
 
 4.4 PPE - Personal Protective Equipment 
 
 
 

[SOP 

 Standard Operating Procedure SOP No Rev 
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 B-909 
 Liquid Encapsulation Procedure 
 
 4.5 CCP — Critical Control Point 
 
 4.6 IPA — 70% Isopropyl Alcohol 
 
 5.0 References 
 
 5.1 A-108, SOP, Good Manufacturing Practices and Personal Hygiene 
 
 5.2 C-707, SOP, Critical Control Point Specifications 
 
 5.3. B-905, SOP, Quality Inspection Process 
 
 5.4 E-803, SOP, Inspection of Palletized In-Process and Finished Product
 
 5.5 B-111, SOP, Cleaning of Manufacturing/Production Areas and Equipment
 
 5.6 G-207, SOP, Calibration Verification and Operation of Scales 
 
 5.7 C-707-F3, Form, CCP2 — Encapsulation Startup Check 
 
 5.8 C-104-F15, Form, Encapsulation Record 
 
 5.9 C-104-F30, Form, Banding Record 
 
 6.0 Procedure 
 
 Ensure that the liquid encapsulation room and scoops are clean and that all cleaning has
6.1 been documented as outlined in SOP B-111 Cleaning of Manufacturing/Production

 Areas and Equipment. 
 
 Ensure that the liquid encapsulation room is clear of all evidence of the previous batch.
6.2 Ensure that scale calibration is verified daily and that the designated log book has been
 
6.3 filled out. Refer to SOP G-207 Calibration Verification and Operation of Scales.

 6.3.1 Check the calibration sticker to ensure that the scale calibration has not expired.
 Report expired calibration to QC personnel and remove from service.
 
 
 

[SOP 

 Standard Operating Procedure SOP No | Rev ae 
 Liquid Encapsulation Procedure B-909 1 age 5 0 
 
 Note: Do not use expired calibration equipment. 
 
 6.4 Identify the liquid encapsulation room with a product detail tag. 
 
 6.5 An inspection of the room, equipment, and utensil cleanliness must take place prior to
 bringing any product into the room. The inspection must be documented in the BPR.
 
 6.6 Prior to the start of a new batch run, the operator and QC inspector must check all
 
 materials and product containers for the following: 
 
 6.6.1 Check the material ID# against the BPR. 
 
 6.6.2 Check the description of material against the BPR. 
 
 6.6.3 Check the batch number against the BPR. 
 
 6.6.4 Ensure that the blended material has been released by QC. 
 
 6.6.5 Ensure that the inner capsule has been released by QC (if applicable).
 
 6.6.6 Ensure that the capsules have been released or approved by QC Laboratory for
 
 at risk use. 
 
 6.6.6.1 An issuance report must be generated prior to room startup which
 outlines the release status of each lot of capsules to be used. QC
 
 Laboratory must approve to use at risk any lot of capsules that are in
 quarantine status. 
 
 6.6.7 The operator must verify that the quantity of material is available for the job.
 
 Note: If any confirmation is not met, notify Operations and Quality
 
 Management. 
 
 6.7. Personnel and visitors must follow GMP as per SOP A-108 Good Manufacturing
 
 Practices and Personal Hygiene. Personnel must follow safety precautions by wearing
 
 appropriate PPE. 
 
 
 

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 Standard Operating Procedure SOP No | Rev 
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 Liquid Encapsulation Procedure 
 
 6.7.1 Personnel must be garbed appropriately with frocks and/or lab coats, hairnets,
 shoe covers and/or dedicated shoes as applicable, beard covers (if applicable),
 
 dust masks, gloves, and safety glasses. 
 
 6.7.2 Operators must change gloves before entering the liquid encapsulation room.
 
 6.8 Liquid Encapsulation 
 
 6.8.1 All encapsulation activities are recorded on form C-104-F15 Encapsulation
 Record and form C-707-F3 CCP-2 Encapsulation Startup. 
 
 6.8.2 Make all necessary adjustments to ensure that the capsules meet the current
 product specifications as outlined in the BPR. 
 
 6.8.2.1 Capsule Weight 
 
 @ Capsule weight ranges are specified in each product profile.
 
 Specifications are as follows unless otherwise specified.
 
 o Minimum Allowed Weight: -10% of target weight 
 
 o _ Lower Control Limit: -5% of target weight 
 
 o _ Upper Control Limit: +5% of target weight 
 
 o Maximum Allowed Weight: +10% of target weight 
 
 6.8.3 Ensure that the correct capsule size, color, and material are used as specified in
 the BPR. 
 
 6.8.4 Ensure that the capsule length is within the required tolerances as outlined in the
 BPR. 
 
 6.8.5 The encapsulation procedure stated in the BPR must be strictly followed. Keep
 encapsulation equipment adjusted to maintain all required specifications.
 
 
 

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 Standard Operating Procedure SOP No | Rev 
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 Liquid Encapsulation Procedure 
 
 6.8.6 Ensure that form C-707-F3 CCP 2 - Encapsulation Startup Check has been
 completed by QC and passes all requirements. Refer to SOP C-707 Critical
 
 Control Point Specifications. 
 
 6.8.7 The operator performs ten capsule checks at startup and every thirty minutes
 until the end of the encapsulation run to ensure product quality.
 
 6.8.8 The QC inspector performs ten capsule checks as outlined in SOP B-905
 Quality Inspection Process to ensure product quality. 
 
 6.8.9 During encapsulation, the following steps should be observed.
 
 6.8.9.1 Record the in-process weights in the BPR. 
 
 Note: Production Management (with evaluation from R&D and/or QC
 Laboratory Management) must approve any instance of weights
 
 running outside of the specified lower and upper control limits.
 
 6.8.9.2 Record the in-process capsule lengths in the BPR. 
 
 6.8.9.3. Ensure that capsules are completely locked with consistent size, color,
 liquid quantity, and inner capsule (if applicable). 
 
 6.8.9.4 Document in the BPR if adjustments are made. 
 
 6.9 Banding 
 
 6.9.1 All banding activities are documented on form C-104-F30 Banding Record.
 
 6.9.2 Make all necessary adjustments to ensure that capsules meet the current product
 
 specification as outlined in the BPR. 
 
 6.9.2.1. Ensure appropriate banding glue is applied as specified in the BPR.
 
 6.9.2.2 Ensure the appropriate banding components are used as specified in
 
 BPR. 
 
 
 

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 Standard Operating Procedure SOP No | Rev 
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 Liquid Encapsulation Procedure 
 
 6.9.3 During the banding process, the following steps should be observed:
 
 6.9.3.1 Ensure the banding glue is changed per the hours specified in the BPR.
 
 6.9.3.2 Ensure the capsules are banded correctly and consistently.
 
 6.9.3.3 Check capsules in the vacuum chamber as specified in the BPR. Notify
 
 department supervisor/manager of any leaks. 
 
 6.9.3.4 Document band adjustments, when applicable. 
 
 6.9.4 Hold Time 
 
 6.9.4.1 After the banding process, capsules are placed on cleaned/lined trays
 
 for drying. Follow and document the hold times as specified in the
 
 BPR. 
 
 6.9.4.2 Trays/racks must be cleaned per SOP B-111 Cleaning of
 
 Manufacturing/Production Areas and Equipment. Trays are to be lined
 with FDA approved food contact butcher paper. Butcher paper must be
 
 disposed after each batch. When the butcher paper is not in use, it must
 be covered appropriately to prevent cross contamination.
 
 6.9.4.3 Once the capsules have met the required hold time, they will be
 
 transferred to a holding container. 
 
 6.9.4.4 Verify that the in-process containers are identified with an in-process
 
 label that includes the following information: 
 
 e Product Name 
 
 ® Batch Number 
 
 ® Container Number 
 
 e Weight per Container 
 
 
 

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 Standard Operating Procedure SOP No Rev 
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 Liquid Encapsulation Procedure 
 
 6.9.5 Completion of the encapsulation process requires the following steps.
 
 6.9.5.1 Place the filled containers with capsules onto the floor scale and record
 
 the container weight in the BPR. 
 
 Note: All production holding drums MUST be wiped down with IPA on the
 
 outside of the drum and lid to remove excess product prior to removing
 
 from the encapsulation area. 
 
 6.9.5.2 Complete the yield calculation and reconciliation in the BPR and move
 
 all in-process containers to the in-process area. 
 
 6.9.5.3 The pallets should be properly identified by a PITT that includes the
 
 product name, customer name, batch number, pallet number(s), and
 quantity. Refer to SOP E-803 Inspection of Palletized In-Process and
 
 Finished Product. 
 
 6.9.6 After encapsulation, cleaning of the room and equipment must be performed
 according to the appropriate procedures and documented as outlined in SOP
 
 B-111 Cleaning of Manufacturing/Production Areas and Equipment.
 
 7.0 Revision History 

| Rev | Date | Description of Changes | CCR # | By |
|-----|----------|------------------------|-------|----|
| 0 | 08/16/21 | New procedure. N/A R. Petrovski Updated procedure to reflect current practices. Added additional Nee d s e e f c i t n i i o t n i 6 o . n 9 s . a 3. n d R e r v e i fe s r e e d n c i e n s s . p e U c p t d i a on t e f d r e f q o u r e m nc a i t e a s. n d A d lo d g e o. d A b d an d d e i d n g to Clea ete Ke Blozqusky | - | - |

 hold time documentation requirements.