Laboratory Housekeeping

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4.00 Definitions 
 Ay 1 Warnes) = Giassware, plasticwane,, and metalware inclusively 
 
 422 IPA= Isopropyl Alcohol 
 433 QE = Quality Control 
 
 500 Referemes 
 
 511 D-824, SOP, Operation and Cleaning of the M1 1 Autoclave 
 22 D-603, SOP, Hazardous Chemical Waste Disposal 
 
 600 Procedure 
 611 Ongoing Cleanliness 
 
 61111 Cleanlimess = When practical, alll associated buffers, samples and wares will be
 held wimtil after the experiment is complete and results have been reviewed. All
 personnel should clean up after the closing ofeach experiment. Dirty wares can
 be presoaked in a wash basin and cleaned at any time up until the end oftthe day.
 
 
 

[SOP 

 Standard Operating Procedure SaOpP aNo Re4 v | Page 2 of 3
 
 Laboratory Thusekeeping 
 
 61122 Throughout the day, proper disposal of laboratory waste, wiping contamimated
 surfaces, and retuming unused reagents and supplies to their proper storage area
 should be practiced fastidiously. 
 
 61133 Equipment usage = Instrumemits need to be properly cleaned, shut down, and/or
 put on stamdipy after each use when appropriate. Instrument surfaces should be
 maintained free of any dust or chemical residue. 
 6.1133.1 HPLC colunmms sinoulld be washed them preserved im am appropriate
 storage buffer before removing from the system. 
 
 6.11322 When applicable, lamps should be tumed off if they are not being used
 for short periods (i.e. overnight) and equipment should be shut down as
 applicable for extended periods (i.e. weekends and holidays).
 611333 The biological containment hood will be samitized before each use by
 asapiicdlly wiping intemal surfaces with, 70% IPA and drying before
 use. At the end of each use, the surfaces willl be sanitized by wiping
 
 with 70% IPA, closing the hood to tum off the blower, then activating
 the UV lamp for 15 minutes. 
 611334 Cleaning of the autoclave is outlined im SOP D-824 Operation and
 Cleaning of the MI 1 Autoclave. 
 
 61144 Trash/irefuse needs to be disposed ofas quiickly as possible after generation. All
 non-shamps and non-biological materials can be disposed of in general waste.
 6.114411 Sharps, such as needles and scalipels, must be disposed of in a sharps
 container. 
 
 611422 Broken glass ant otiner brokem items with strap edges sinould be
 disposed of in am appropriately fitted amd labeled box designated for
 broken glass. 
 611443 Potentidliy hazardous biological waste sinoulld be disposed! of im an
 appropriately fitted amd labeled box designated as a biological waste
 container. Tike comtdinars and contents shoddd be suiitdibte for
 
 incineration. 
 6.11444 All other hazardous wastes will be handled and disposed of as outlimed
 in SOP D-603 Hazardous Chemical Waste Disposal. 
 
 6.2 End of the Day Routime 
 6211 When appropriate, at the end of each watk day, bench tops amt] equipment
 should be inspected amd cleaned to ensure surfaces are free of dust or chemical
 residue. The wastibasin shouid be dear Of all usat glasswexe anti clean
 glassware should be dried before retuming to storage. 
 
 621111 Used glassmrae amd plasticware cam be soaked ovenmight im a wash
 basin. 
 62122 Clean wares must be removed from the wash basin, cleamad) and
 allowed to dry before returning to storage daily. 
 
 
 

[SOP 

 Standard Operating Procedure SOP No Rev 
 D-101 4 Page 3 of 3 
 Laboratory Housekeeping 
 
 33 Weekly Cleaning 
 63311 The @€ Laboratory floors should be cleaned weekly at a minimum using a mop
 
 and an approved cleaning solution. 
 G4 
 General Practices 
 644111 Food, beverages, gum, candy, breath mints, or tobacco products are not allowed
 in the laboratory areas at any time. 
 6422 Lab safety eyewear at a mininmum are required when working in the lab.
 
 64833 All signage must be followed whem entering the lalb or lay subsections (i.e
 micro.) 
 6444 If performing tests that require specialized PPE, the PPE required must be wom
 while the experiment is in process. Other individuals that are working in the
 surrounding areas sinoulld be notified of amy risks associated with exposure and
 may need to wear appropriate PPE as well. 
 
 64455 Laboratory coats exposed to laboratory chemicals should be processed through
 the laundry service immediately. 
 700 Revision History 
 
 Revision Date Desoription ofChanges CCR # By 
 
 0 @5/06t10 | New “ = 
 ] O2O#N3 | Updated SOP format. Made SOP more detailed. 13-032 B. Johns 
 Updated SOP format. Expanded responsibilities. Added reference 
 2 01/07/15 | to D-824 and D-603. Added cleaning oflbiological cabinet. 15-0037 B. Johns
 Increased detail ofcizaning process. Biennial Review. 
 3 04/04/17 | Biennial review. Updated respomsibilitizs. 7-035 | B. Johns
 4 04/1 8120 Scheduled review, added safety infommatiom, following, alll signage €E206236 | I.Maignan
 and wearing safety glasses while in the lab