QC Laboratory Glassware Cleaning

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1.0 Purpose 
 
 The purpose of this procedure is to provide guidelines for cleaning laboratory wares for use in
 
 the QC Laboratory. 
 
 2.0 Scope 
 This procedure applies to all reusable wares used in testing or experiments in the QC
 
 Laboratory. This procedure does not cover disposable wares or sterilization.
 
 3.0 Responsibility 
 
 4.1 Itis the responsibility of QC analysts to follow this procedure, 
 
 3.2 It is the responsibility of QC Laboratory Management to implement this procedure and
 to ensure that the procedure is being followed. 
 
 33 It is the responsibility of QC Laboratory Management to keep this procedure current
 
 with latest Ion Labs practices. 
 
 4.0 Definitions 
 
 4.1. Ware(s) — Glassware, plasticware, and metalwate inclusively 
 
 42. IPA- Isopropyl Alcohol 
 
 43 MeOH - Methanol 
 
 4,4 QC - Quality Control 
 
 
 

[SOP D-104 | Page 2 of 4]

 QC L S a t b an o d r a a r t d o O r p y e G ra l t a i s n s g w a Pr r o e c C e l d e ur a e n ing S D O - P 1 0 N 4 o | Re 5 v Pave? age 2 o o f f 4
 
 5.0 References 
 
 5.1 D-824, SOP, Operation and Cleaning of the Tuttnauer EZ10 and 3850EA Autoclaves
 
 6.0 Procedure 
 
 6.1 Detergent Selection 
 
 6.1.1 Only detergent labeled for use with laboratory wares will be used. Micro-90
 
 Concentrated Cleaning Solution (Item: M-9050-12) will be used as the first
 option when available. For specialty cleaning, an appropriate detergent will be
 
 selected that is compatible with the surface and cleaning method.
 
 6.2 Cleaning of Wares 
 
 6.2.1 A fresh soap solution should be made at least once weekly and replaced as
 needed. Soap should be diluted as per manufacturer instructions.
 
 6.2.2 Prerinse items with tap water and remove all loose material from the wares
 before placing into soap solution. If there is a residue that is especially resistant
 
 to removal, the glassware may be presoaked separately using an appropriate
 detergent to loosen the residue before continuing with general cleaning. An
 
 ultrasonic cleaner may also be used to remove residue that is not easily reached.
 
 6.2.3 When soaking is necessary, items should be completely submerged and interiors
 
 completely filled with detergent solution. Wares should be soaked for a time
 
 sufficient to loosen or remove all residue and deposits. Metal components
 should not be soaked for more than 24 hours as corrosion may occur.
 
 62.4 After the item has been soaked, inspect glassware for markings and labeling
 which should be removed, Acetone, Methanol, and IPA can be used to remove
 
 ink and label glue. Used organic solvents must be stored in a waste solvent
 
 container for disposal. 
 
 6.2.5 Rinse wares with tap water and a final rinse with Millipore water before drying.
 
 
 

[SOP D-104 | Page 3 of 4]

 QC L S a t b an o d r a a r t d o O r p y e G ra l t a i s n s g w a Pr r o e c C e l d e ur a e n ing S D O - P 1 0 N 4 o | Re 5 y Pave age 3 3 of4
 
 6.2.6 Dry items in an oven with temperature adjusted between 35°C and 45°C
 
 (suggested). 
 
 6.2.7 Items can also be allowed to air dry at room temperature. 
 
 6.2.8 For dried wares that require sterilization, refer to SOP D-824 Operation and
 
 Cleaning of the Tuttnauer EZ10 and 3850EA Autoclaves. 
 
 6.3 Cleaning of Pipettes 
 
 6.3.1 Fill the pipette station with tap water and detergent. 
 
 6.3.2 Insert the pipette basket loaded with pipettes to be cleaned. Pipette tips should
 
 all be facing up. 
 
 6.3.3 Soak the pipettes for 2 to 24 hours in detergent. 
 
 6.3.4 Transfer the pipette basket to the pipette rinse station. 
 
 6.3.5 Fill the rinse station with tap water and then Millipore water, then drain.
 
 6.3.6 Repeat step 6.3.5 as necessary to remove detergent residue. 
 
 6.3.7 Fill the rinse station with Millipore water for the final rinse.
 
 6.3.8 Pipets can also be cleaned by forcing water and detergent through with an
 appropriate spray bottle or other means. Detergent residue should be removed
 
 taking care to ensure water is moved sufficiently through the inside walls of the
 
 small aperture of the pipets. 
 
 
 

[SOP D-104 | Page 4 of 4]

 Standard Operating Procedure SOP No | Rev Pave 4 of 4 
 QC Laboratory Glassware Cleani * ng D- se 104 5 ie 4 6: 
 
 7.0 Revision History 

| Rev | Date | Description of Changes | CCR # | By |
|-----|----------|------------------------|-------|----|
| 0 | 05/06/10 | New I 02/04/13 tUhped austee do fS cOlPea nfionrgm/astt.e rIimlipzraotvioend eSquOiPp maenndt .a dded information on 13-033 B, Johns | - | - |
| 2 | 01/20/15 | w U a p t d e a r t e w d i t S h O t P ap f w o a r t m e a r t . , R E e x p p l a a n c d e e d d T r y e p s e po n { s w i a bi t l e i r t i w e i s t , h R M e i p l l l a i c p e o d r e f i w l a t t e e r r e . d | 15-0054 | B. Johns |
| 3 | 04/04/17 | C Bi h e a n n n g ia e l r r e e sp v o i n e s w i . bilities to meet current practices. Clarified wording. Biennial review. | 17-0347 | B. Johns |
| 4 | 04/15/20 | 2 Sc x h r e e d q u u l i e r d e m R e e n v t i s e f w o ; r a r d in d s e e d s. a R lt i e n r s n e a s t i c v a e n c b le e a p n e i r ng f o f r o m r e p d i p a e s t s m , a r n e y m oved CC- | 20-0297 | J, Maignan |
| 5 | 03/03/22 | U t e a c e nt. CC-22-0088 J. Sassnan | - | - |