D-306

Finished Product Qualification and Reduced Testing

Section D — Laboratory Operations and Specifications Revision 3 11 pages

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1.0 Purpose 
 
 The purpose of this procedure is to define the Product Qualification process that allows for a
 
 reduced testing plan, which includes a full, routine, or periodic testing regime of dietary
 
 supplement products manufactured at Ion Nutritional Labs manufacturing facilities, while still
 ensuring that the finished dietary supplement product meets all specifications for identity,
 
 purity, strength, composition, and impurity limits. 
 
 2.0 Scope 
 
 This procedure applies to all dietary supplement formulations, pet products, and foods
 
 produced by Ion Nutritional Labs. The Jon Nutritional Labs criteria for reduced testing can be
 superseded with a testing program provided by the customer. 
 
 3.0 Responsibility 
 
 3.1 ‘It is the responsibility of QC Chemists to test finished products for release and to
 confirm test results. 
 
 3.2 It is the responsibility of QC Laboratory Management and R&D to determine risk level
 to select which formulations are eligible for reduced testing. 
 
 3.3. QC and R&D Management will determine the testing schedule and generate tracking
 sheets which predefine the testing to include full, routine, and periodic.
 
 3.4 ‘It is the responsibility of QC and DC to maintain all required documentation in support
 of the reduced testing program. 
 
 
 

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 3.5 It is the responsibility of DC to maintain copies of the product profiles with the reduced
 testing exemptions and eligibility exceptions for reduced testing.
 
 4.0 Definitions 
 
 4.1 QC — Quality Control 
 
 4.2 R&D — Research and Development 
 
 4.3 DC — Document Control 
 
 4.4 Certificate of Analysis (CofA) — A formal laboratory-prepared document that details
 
 the results and analytical methods for one or more laboratory analyses
 
 4.5 Certificate of Compliance (CofC) — A formal document which certifies that the goods
 
 supplied meet the required standards 
 
 4.6 Full lot test — a test of a lot for all product attributes that might occur that are not either
 
 a qualification or a requalification (4.e. annual, periodic, or formula change)
 
 4.7 TYPE 1 Stability — to establish the expiration interval of a formulation or modified
 formula 
 
 4.8 Qualification — the process of testing all attributes of a lot when a product is first
 developed or modified or for a product’s annual full test 
 
 4.9 Requalification — the process of verifying the qualification of a product after a
 confirmed variance in the product is observed. There may be a change in the product
 
 formulation to correct for the observed variance. 
 
 4.10 Optimal Testing Frequency — a calculation derived from the number of lots produced
 during a three year cycle (N) using the formula (square root of N + 1) to determine the
 
 number of lots that are not full tested before a lot is full tested again
 
 4.11 NFP — Nutritional Facts Panel 
 
 
 

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 4.12 Rotation Schedule — a product’s testing rotation of active ingredients for each lot based
 on the optimal testing frequency calculations and other factors 
 
 4.13 Minimum Quality Control (MQC) — the minimum testing that must be conducted on
 
 each lot which accomplishes the most amount of reduction in attribute testing
 
 4.14 Full Testing — all active ingredient tests including other testing parameters are
 
 conducted 
 
 4.15 Reduced Testing — all active ingredients are tested with other testing parameters but
 
 testing is divided over consecutive lots, with each lot being tested for a minimum of one
 active ingredient 
 
 4.16 Routine Testing — all active ingredients are tested with other testing parameters but
 testing schedule is divided over consecutive lots and every other lot gets tested at least
 
 one active ingredient test (minimum quality control testing applies to this test regime)
 
 4.17 Periodic Testing — all active ingredients are tested with other testing parameters but
 some lots may not have any active ingredient tests depending on the calculated test
 
 frequency (minimum quality control testing applies) 
 
 4.18 Skip Lot Testing — all active ingredient tests are conducted over a subset of lots
 
 produced (minimum quality control testing does not apply) Note: This is not considered
 a reliable testing plan and will only be applied by customer request
 
 5.0 References 
 
 5.1 21 CFR 111.75 - What must you do to determine whether specifications are met?
 
 5.2 21 CFR 111 subpart M — Holding and Distributing 
 
 5.3 D-401, SOP, Finished Product Testing and Specification Requirements
 
 5.4 D-902, SOP, Establishment of Specifications 
 
 
 

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 5.5 ICH Q7 — Good Manufacturing Practices for Active Pharmaceutical Ingredients
 document 
 
 5.6 21 CFR 101.9 — Nutrition Labeling for Food 
 
 6.0 Procedure 
 
 6.1 Product Qualification: 
 
 6.1.1 Products manufactured in-house at Ion Nutritional Labs facilities will be
 qualified by verifying that using a specific set of raw materials evaluated in
 
 accordance to the Raw Material Testing Summary, warehouse controls, a master
 
 formula, master batch record, manufacturing controls, and quality management
 systems to ensure that the dietary supplement product consistently meets the
 
 established product specifications. The warehouse controls are set in place to
 comply with warehouse or other storage facilities holding a dietary supplement
 
 as described in subpart M in FDA 21 CFR 111. The requirements for release
 
 are that every finished batch of dietary supplement meets each product
 specification for identity, purity, strength, composition and limits on
 
 contamination that may adulterate a finished product produced at Ion Nutritional
 Labs as per 21 CFR 111.75. 
 
 6.1.2 Following best practices outlined by the ICH Q7 Good Manufacturing Practices
 for Active Pharmaceutical Ingredients document, the first three (3) qualification
 
 production lots of a product are fully evaluated for identity, purity, strength,
 
 composition and limits on potential contaminants per the Finished Product Test
 Ticket. 
 
 6.1.3 Specifications for the Nutritional Supplemental Facts as defined in 21 CFR
 101.9, Nutrition Labeling of Food, which includes calorie, total fat, cholesterol,
 
 total carbohydrates, fiber and sugars will be established based on calculation and
 then evaluated as report only on the first production lot of the product as a part
 
 of the product qualification. 
 
 
 

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 6.1.4 The minimum required identification testing includes organoleptic analysis,
 macroscopic analysis and at least one chemical analysis or other scientifically
 
 valid method to definitively identify the material. Identification is considered a
 
 Minimum Quality Control (MQC) parameter and will be tested on every single
 lot regardless of testing program established for release. 
 
 6.1.5 The minimum required purity testing includes Microbiological Purity specified
 for the product unless the product has been placed on an alternate testing
 
 schedule. Justification for routine or periodic testing is based on risk assessment
 
 using historical analysis, biocidal status, and other factors that may impact
 microbiological purity of the product. 
 
 6.1.6 The minimum required physical parameter QC laboratory testing includes unit
 average weight, weight variation, density, pH, viscosity, total dissolved solids,
 
 and other physical parameters are examined as specified for the product. All
 physical property parameters are considered MQC and will be tested on every
 
 lot unless otherwise specified by the customer. 
 
 6.1.7 Following the successful completion of the qualification lots, a full, routine, or
 
 periodic testing schedule may be employed for all parameters not covered under
 
 MQC. 
 
 6.1.8 Unless otherwise specified by the customer, product contamination testing
 
 which may include elemental impurities, pesticides, residual solvents,
 melamine, gluten, peroxide value, acid value, various toxins will be reduced to
 
 an annual evaluation provided the data from the product qualification support
 
 the reduced testing practice. Vendor incoming raw material CofA’s should be
 screened routinely for contaminant limits to ensure the materials are suitable for
 
 use in manufacturing. 
 
 
 

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 6.1.8.1 In instances where the product is shipped to a region or country where
 certain contaminants are tested on every lot, the Finish Product Test
 
 Ticket will define the periodicity of the contaminant testing.
 
 6.2 Routine Rotational Testing: 
 
 6.2.1 A routine rotational schedule begins after three (3) lots are fully tested. A risk
 
 assessment is performed after the three lots are completed to confirm the
 product is eligible. Confirmed failures of any specified test, reformulation
 
 during the test period and other changes or confirmations can lead a product
 being ineligible for a routine rotational schedule until additional data is collected
 
 to confirm the robustness of the formula and manufacturing process. This type
 
 of rotational testing can be selected by the customer. 
 
 6.2.2 The testing frequency for a routine rotational schedule can vary between less
 
 than full testing and testing a subset of ingredients in a rotation. Testing
 frequency can be customer defined with consideration of the product risk.
 
 6.2.3 The testing for successive lots will rotate through the subsets of active
 ingredients sequentially until all active ingredients tests have been completed
 
 before the rotational schedule repeats. The rotational selection is predefined and
 
 can only be changed by events such as a confirmed out of specification or a
 change in formulation, both of which require the requalification of the product.
 
 6.2.4 A minimum of one annual full testing is required for each calendar year. A full
 testing cycle can be substituted for any rotation cycle in the routine rotational
 
 schedule at any time. 
 
 6.3 Periodic Rotational Testing: 
 
 6.3.1 A periodic rotational schedule begins after three (3) lots are fully tested. A risk
 
 assessment is performed after the three lots are completed to confirm the
 product is eligible. Confirmed failures of any specified test, reformulation
 
 
 

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 during the test period and other changes or confirmations can lead a product to
 being ineligible for a periodic rotational schedule until additional data is
 
 collected to confirm the robustness of the formula and manufacturing process.
 
 This type of rotational testing can be selected by the customer. If in house
 stability testing is performed, at least one eligible stability batch will have all
 
 analyzable markers tested when the markers are required as a part of a Type 1
 Stability assessment. 
 
 6.3.2 The testing frequency for a periodic rotational schedule can vary between less
 
 than every other active ingredient to testing a subset of ingredients in a rotation
 with MQC only testing performed at different frequencies between each subset
 
 of ingredient testing. Testing frequency is defined considering product risk and
 can be customer defined. The periodic testing frequency is typically less than
 
 the routine rotational frequency. 
 
 6.3.3 The testing of successive lots will rotate through the subsets of active
 
 ingredients sequentially until all active ingredients tests have been completed
 
 before the rotational schedule repeats. The rotational selection is predefined and
 can only be changed by events such as a confirmed out of specification or a
 
 change in formulation, both of which requires the requalification of the product.
 
 6.3.4 A minimum of one annual full testing is required for each calendar year. A full
 
 testing cycle can be substituted for any rotation cycle in the routine rotational
 schedule at any time. 
 
 6.4 Rotation Schedule Examples: 
 
 6.4.1 Table 1 provides an example of how the routine rotational schedule would
 
 proceed for a hypothetical product that has three active ingredients (AI) and is
 
 eligible for routine testing with a customer request of at least one label claim
 ingredient (LCI) tested every lot: 
 
 
 

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 Table 1: 
 Lot# of Formula SCTOOXXX Testing required 
 1 451230 Full Testing: all LCI, NFP and MQCs 
 2TM4 451240 Full Testing: all LCI and MQCs 
 3TM 451250 Full Testing: all LCI and MQCs 
 4'" 451260 Reduced testing: Al with MQCs 
 5 451270 Reduced testing: A2 with MQCs 
 6" 451280 Reduced testing: A3 with MQCs 
 
 7" 451290 Reduced testing: Alwith MQCs 
 8" 451300 Reduced testing: A2 with MQCs 
 NFP = Nutritional facts panel. MQC = Minimum quality control 
 
 6.4.2 Table 2 provides an example of how the periodic rotational schedule would
 
 proceed for a hypothetical product that has three active ingredients (AI) and is
 eligible for periodic testing assuming the risk assessment with customer
 
 approval calls for a ratio of testing 1 lot, skip rotate 3 lots:
 
 Table 2: 
 Lot# of Formula SCTOOOXXX Testing required 
 1 451230 Full Testing: all LCI, NFP and MQCs 
 2TM 451240 Full Testing: all LCI and MQCs 
 
 3TM 451250 Full Testing: all LCI and MQCs 
 4'" 451260 Reduced testing: Al with MQCs 
 
 5 451270 Reduced testing: MQCs only 
 6" 451280 Reduced testing: MQCs only 
 7" 451290 Reduced testing: MQCs only 
 
 8" 451300 Reduced testing A2 with MQCs 
 9" 451310 Reduced testing: MQCs only 
 
 10" 451320 Reduced testing: MQCs only 
 11 451330 Reduced testing: MQCs only 
 12" 451340 Reduced testing A3 with MQCs 
 
 13th 451350 Reduced testing: MQCs only 
 14" 451360 Reduced testing: MQCs only 
 15" 451370 Reduced testing: MQCs only 
 
 16th 451380 ( Repeat cycle) Reduced testing: Al with MQCs 
 LCI = Label Claim Ingredients NFP = Nutritional facts panel. MQC = Minimum quality control
 
 
 

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 6.5 Full lot, Qualification and Requalification Testing: 
 
 6.5.1 A full lot test of the product which includes a complete evaluation for identity,
 
 purity, strength, composition and limits on potential contaminants as per the
 
 Finished Product Test Ticket is conducted. 
 
 6.5.1.1 Once annually for all products regardless of reduced testing program
 
 implemented. 
 
 6.5.1.2 Once every 20" lot with routine testing and once every 30" lot with
 
 periodic testing for all products with 100 production lots per year is the
 default requirement. Products with less than 100 production lots
 
 require only annual. Periodic full testing cycles can be defined by
 
 Optimal Test Frequency calculations and by customer requirement.
 
 6.5.2 Qualification is performed when a new formula has started production. A
 
 complete evaluation for identity, purity, strength, composition and limits on
 potential contaminants as per the Finished Product Test Ticket is performed.
 
 6.5.2.1 The first lot in the series for the qualification requires NFP testing if
 the label contains a nutritional facts panel. 
 
 6.5.3 Requalification includes a complete evaluation for identify, strength, purity,
 composition and limits on contaminants as per the Finished Product Test Ticket.
 
 Requalification is performed when: 
 
 6.5.3.1 A master formulation change has been made that involves an
 ingredient composition adjustment or a significant change to the
 
 manufacturing process. Requalification using full testing for a
 minimum of the first three lots is required. 
 
 6.5.3.2 A confirmed out of specification incident occurs due an unexplained
 variation or no determined root cause. A Material Review Board
 
 (MRB) review and disposition of the lot and corrective actions will be
 
 
 

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 performed to determine if requalification is required. A minimum of
 three lots are required to requalify a product. 
 
 6.5.3.3 Once annually as a best practice standard. 
 
 6.5.4 Exceptions where requalification is not required: 
 
 6.5.4.1 Out of Specification (OOS) result for a contaminant when it is not
 
 attributed to a manufacturing process that requires a significant change
 to eliminate the contamination risk. 
 
 6.5.4.2. OOS result where the root cause traces back to a raw material (RM)
 issue when correction of the RM issue eliminates the risk to the
 
 product. 
 
 6.5.4.3 A failure in an Ingredient Addition Verification (IAV) calculation that
 
 traces back to an RM or production issue when correction of the issue
 
 eliminates the risk to the product. 
 
 6.5.4.4 The decision to requalify must be reviewed and approved by Quality
 
 Control management and must align with customer requirements.
 
 6.6 Documentation: 
 
 6.6.1 A tracking and data archiving system will be maintained which shows at
 minimum, the projection of the reduced testing cycle, the completed lots for
 
 testing, and which tests were performed, and the test results.
 
 6.6.2 A reference to the master formulation under which the qualification was
 
 performed. 
 
 6.6.3 Identification of the minimum three or more lots used for the qualification.
 
 6.6.4 Identification of lots used for requalification. 
 
 
 

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 6.6.5 Identification of the annual requalification lot. 
 
 6.6.6 Electronic copies of the approved Finished Product Test Tickets for the lots
 
 evaluated for any level of qualification or requalification. 
 
 6.6.7 Maintain copies of OOS records evaluation of risk when implementing reduced
 
 testing. 
 
 6.6.8 Based on customer requirements, COAs and COCs can be generated as a part of
 the batch release to provide assurance that the product meets all quality and
 
 safety requirements. 
 
 7.0 Revision History 

| Rev | Date | Description of Changes | CCR # | By |
|-----|----------|------------------------|-------|----|
| 0 | 02/08/16 | New | 15-1093 | B. Johns |
| 1 | 08/23/16 | Add criteria for customer requested reduced testing. Added reduced testing requirements for liquids. | 16-0758 | B. Johns |
| 2 | 12/02/19 | 3 year review. Minor clarification. | 19-0912 | J. Sassman |
| 3 | 07/01/24 | Complete rewrite of the program. CC- | 24-0322 | B. Johns |