D-777

Elemental Analysis by ICP-MS

Section D — Laboratory Operations and Specifications Revision 8 37 pages

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1.0 Purpose 
 
 This procedure provides guidelines for the elemental analysis of raw materials and finished
 
 products using ICP-MS. 
 
 2.0 Scope 
 
 This procedure applies to the testing of label claim elements and elemental impurities in finished
 
 products and raw materials in the QC laboratory at Ion Labs. The ICP-MS method for the
 elemental analysis of raw materials and finished products has two functions:
 
 rae | The measurement of elemental impurities including, but not limited to, As, Ag, Au, Ba,
 
 Cd, Co, Cr, Cu, Hg, Ir, Li, Mo, Ni, Os, Pb, Pd, Pt, Rh, Ru, Sb, Se, Sn, Tl, V.
 
 2.2 The measurement of macro-elements associated with label claims including, but not
 
 limited to B, Ca, Cr, Cu, Fe, K, Mg, Mn, Mo, Na, P, Se, Sr, V, and Zn.
 
 3.0 Responsibility 
 
 3.1 It is the responsibility of QC Laboratory Management to ensure that elemental analyses
 
 are performed in accordance with the procedure described herein. 
 
 3.2 It is the responsibility of Analytical Development personnel and/or QC Laboratory
 Analysts to perform routine analyses and method validations. 
 
 3.3 It is the responsibility of Analytical Development personnel and/or QC Laboratory
 
 Management to determine if a product specific method validation is required for products
 being analyzed for the first time using this method. 
 
 
 Odate ever ATS G3l0vlzs 

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 3.4 It is the responsibility of the Analytical Development personnel and/or QC Laboratory
 Management to keep this procedure current with the latest lon Labs Practices.
 
 4.0 Definitions 
 
 4.1 PDE — Permitted Daily Exposure for individual elemental impurities. For drug products
 and raw materials used in drug products, these values are typically taken from USP
 
 <232>. For dietary supplements, dietary ingredients, cosmetics, and raw materials used
 in cosmetics, USP <2232> and California Proposition 65 apply. The specifications listed
 
 in raw material or finished product profiles may differ from and always supersede USP
 
 or California Proposition 65 guidelines due to product specific or customer requirements.
 
 Daily Dose PDE (#2) = PDE (#2) = Max Daily Dose (4) 
4.2 4.3 J — The Daily Dose PDE must be corrected for the dilution factor and sample amount to

 calculate the limit of each elemental impurity in the final prepared sample (J). For
 example, the oral PDE for lead is 5 g/day. If the maximum daily dose is 10 g/day, the
 
 sample amount is | g, and the dilution factor is 100 mL/sample, then J is calculated by:
 
 posts 10% 12 = 100 mL x 10n =0 50n—— 
 day day sample sample Lg mL 
 
 4.4 ICP-MS -— Inductively Coupled Plasma — Mass Spectrometry 
 
 4.5 QC — Quality Control 
 
 4.6 AD - Analytical Development 
 
 4.7 EI — Elemental Impurities 
 
 4.8 LC — Label Claim 
 
 4.9 BEC — Background Equivalent Concentration 
 
 4.10 Use Class — All finished products and raw materials manufactured at Ion Labs can be
 
 categorized into four basic classes based on their intended use: 
 
 
 

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 4.10.1 Drug — The Federal Food, Drug, and Cosmetic Act (FD&C Act) and FDA
 regulations define the term drug as: 
 
 4.10.1.1 A substance recognized by an official pharmacopoeia or formulary.
 
 4.10.1.2 A substance intended for use in the diagnosis, cure, mitigation,
 
 treatment, or prevention of disease. 
 
 4.10.1.3 A substance (other than food) intended to affect the structure or any
 
 function of the body. 
 
 4.10.1.4 A substance intended for use as a component of a medicine but not a
 device or a component, part or accessory of a device.
 
 4.10.1.5 Biological products are included within this definition and are
 
 generally covered by the same laws and regulations, but differences
 exist regarding their manufacturing processes (chemical process
 
 versus biological process.) 
 
 4.10.2 Dietary Supplement — The Dietary Supplement Health and Education Act of
 
 1994 (DHSEA) defines a dietary supplement as a product taken by mouth that
 contains a "dietary ingredient" intended to supplement the diet. Dietary
 
 supplements can also be extracts or concentrates, and may be found in many
 forms such as tablets, capsules, softgels, gelcaps, liquids, or powders. They can
 
 also be in other forms, such as a bar, but if they are, information on their label
 
 must not represent the product as a conventional food or a sole item of a meal
 or diet. 
 
 4.10.2.1 Dietary Ingredient — DHSEA defines a dietary ingredient as a
 
 component of a dietary supplement. A dietary ingredient must be one
 or any combination of the following: a vitamin, a mineral, an herb or
 
 other botanical, an amino acid, a dietary substance for use by man to
 
 supplement the diet by increasing the total dietary intake, or a
 concentrate, metabolite, constituent or extract. 
 
 
 

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 4.10.2.2 New Dietary Ingredient — A new dietary ingredient is a dietary
 ingredient that was not sold in the U.S. before October 15, 1994.
 
 4.10.3 Cosmetic — The FD&C Act defines cosmetics as "articles intended to be rubbed,
 
 poured, sprinkled, sprayed on, introduced into, or otherwise applied to the
 human body...for cleansing, beautifying, promoting attractiveness, or altering
 
 the appearance." Included in this definition are products such as_ skin
 
 moisturizers, perfumes, lipsticks, fingernail polishes, eye and facial makeup
 preparations, shampoos, permanent waves, hair colors, toothpastes, and
 
 deodorants, as well as any material intended for use as a component of a
 cosmetic product. 
 
 4.10.4 Food — Food is any substance that is usually composed of carbohydrates, fats,
 
 proteins and water. It can be eaten or drunk by any animal including humans
 
 for nutrition or pleasure. Most of the foods are of plant or animal origin.
 
 5.0 References 
 
 5.1 USP <232> Elemental Impurities - Limits 
 
 Dud USP <233> Elemental Imputities - Procedures 
 
 5.3 USP <2232> Elemental Contaminants in Dietary Supplements 
 
 5.4 California Proposition 65 — Safe Drinking Water and Toxic Enforcement Act of 1986
 
 5.5 21 U.S. Code Sections 321-3991 — Federal Food, Drug, and Cosmetic Act
 
 5.6 U.S. Department of Health and Human Services, National Institutes of Health, Office of
 
 Dietary Supplements, Dietary Supplement Health and Education Act of 1994.
 
 5./ D-105, SOP, Out of Specification / Out of Trend Investigation 
 
 5.8 D-825, SOP, Calibration, Verification and Operation of a Multiwave Go Microwave
 System 
 
 5.9 D-777-F 1, Form, ICP Intermediate Standard Prep Form 
 
 
 

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 5.10 D-777-F2, Form, ICP Working Standard Prep Form 
 
 3s Ld D-777-F3, Form, ICP Sample Prep Form 
 
 Duke D-777-F4, Electronic Form, ICP Standard Concentration Calculator 
 
 5.13 D-777-F5, Electronic Form, ICP Validation/Sample Preparation Calculator
 
 5.14 D-777-F6, Electronic Form, ICP LC Raw Material Sample Prep Calculator
 
 5.15 D-777-F7, Electronic Form, ICP Dilution Calculator 
 
 5.16 D-777-F8, ICP Data Review Checklist 
 
 5.17 D-118, SOP, Laboratory Quality Assurance Activities Plan (Proficiency Testing)
 
 5.18 A-106, SOP, Documentation Guidelines for cGMP Records 
 
 aul C-501, SOP, Document Control Procedure 
 
 5.20 C-502, SOP, Record Storage, Retention, and Destruction 
 
 6.0 Equipment and Materials 
 
 6.1 Agilent 7800 ICP-MS System with MassHunter software 
 
 6.2 Agilent SPS 4 Autosampler 
 
 6.3 Anton Paar Multiwave GO Digestion System 
 
 6.4 Nitric Acid — 67% - 70% Trace Metal Grade 
 
 6.5 Hydrochloric Acid — 32% - 35% Trace Metal Grade 
 
 6.6 Isopropanol — HPLC Grade 
 
 6.7 Single Element Standards Containing each of the following elements at 1000 ppm: B,
 Ca, Cr, Cu, Fe, K, Mg, Mn, Mo, Na, P, Se, Sr, V, and Zn. Other elements may be required
 
 to support product label claims. 
 
 6.8 10 ppm or 1000 ppm Hg Standard. 
 
 
 

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 6.9 Class 1 Stock Solution (SCP Science Custom Standard Part #AQO-113-205 or equivalent)
 Ag ~ 150 ppm, As = 15 ppm, Cd ~ 5 ppm, Co ~ 50 ppm, Ni ~ 200 ppm, Pb ~5 ppm, Se
 
 ~ 150 ppm, Tl ~ 8 ppm, V ~ 100 ppm, 10% Nitric Acid Matrix 
 
 6.10 Class 2 Stock Solution (SCP Science Part #140-131-111 or equivalent) Au ~ 100 ppm, Ir
 
 ~ 100 ppm, Os ~ 100 ppm, Pd ~ 100 ppm, Pt ~ 100 ppm, Rh ~ 100 ppm, Ru ~ 100 ppm,
 10% Hydrochloric Acid Matrix 
 
 6.11 Class 3 Stock Solution (SCP Science Part #140-131-121 or equivalent) Ba ~ 140 ppm,
 
 Cr ~ 1100 ppm, Cu ~ 300 ppm, Li ~ 55 ppm, Mo = 300 ppm, Sb ~ 120 ppm, Sn ~ 600
 ppm, 5% Nitric Acid + 0.5% Hydrofluoric Acid Matrix 
 
 6.12 Internal Standard Stock Solution (SCP Science Part #140-111-081 or equivalent)
 
 Bi ~ 10 ppm, Ge ~ 10 ppm, In ~ 10 ppm, Li(6) ~ 10 ppm, Sc ~ 10 ppm, Tb = 10 ppm,
 
 Y ~ 10 ppm, 5% Nitric Acid Matrix 
 
 6.13 Tuning Solution Stock (Agilent Part # 5188-6564 or equivalent) Ce ~ 10 ppm, Co ~ 10
 ppm, Li ~ 10 ppm, Tl ~ 10 ppm, Y ~ 10 ppm, 2% Nitric Acid Matrix 
 
 6.14 50-mL Plastic Tubes 
 
 6.15 15-mL Plastic Tubes 
 
 6.16 10-mL Adjustable Auto-Pipet 
 
 6.17 l-mL Adjustable Auto-Pipet 
 
 6.18 200-uL Adjustable Auto-Pipet 
 
 6.19 1000-mL Plastic Graduated Cylinder 
 
 6.20 Plastic Beakers and Bottles for Liquid Transfer and Storage 
 
 7.0 Preparation of Solutions 
 
 Fok Autosampler Rinse Solution Preparation 
 
 Note: Rinse Solution has no expiration. Preparation may be scaled as needed.
 
 (a ee Transfer 910 mL of Water to a suitable container. 
 
 
 

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 7.1.2 |Add 30 mL of Nitric Acid and 60 mL of Hydrochloric Acid. 
 
 7.1.3. Close the container and mix well. 
 
 7.2. 5% Nitric Acid Solution Preparation 
 
 Note: Nitric Acid Solution has no expiration. Preparation may be scaled as needed.
 
 7.2.1. Transfer 190 mL of Water to a suitable container. 
 
 7.2.2 Add10mL of Nitric Acid 
 
 7.2.3. Close the container and mix well. 
 
 we Diluent Preparation 
 
 Note: Diluent has an expiration of six months. Preparation may be scaled as needed.
 
 7.3.1 Transfer 880 mL of water to a suitable container. 
 
 7.3.2. Slowly add 100 mL of Nitric Acid. 
 
 7.3.3. Slowly add 20 mL of Hydrochloric Acid. 
 
 7.3.4 Close the container and mix well. 
 
 7.4 Tuning Solution Preparation 
 
 Note: The Tuning Solution has an expiration of one year. 
 
 7.4.1. Transfer 25 uL of Tuning Solution Stock into a 250-mL plastic container.
 
 7.4.2. Add 240 mL of Water. 
 
 7.4.3, Add 10 mL of Nitric Acid. 
 
 7.4.4 Close the container and mix well. 
 
 7.5 Internal Standard Preparation 
 
 Note: The Internal Standard Solution has an expiration of six months.
 
 TA Transfer 25 mL of Internal Standard Stock Solution to a suitable container.
 
 7.5.2 Add 315 mL of Water. 
 
 
 

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 7.5.3. Add 10 mL of Nitric Acid. 
 
 7.5.4 Add 150 mL of Isopropanol. 
 
 7.5.5. Close the container and mix well. 
 
 8.0 Standard Preparation 
 
 8.1 Preparation of E] and LC Intermediate Standard Solutions is documented on Form D-
 
 777-F1 ICP Stock Standard Preparation Form. 
 
 8.2 Preparation of EI and LC Working Standard Solutions is documented on Form D-777-F2
 ICP Working Standard Preparation Form. 
 
 8.3 Electronic Form D-777-F4 is used to calculate the concentration of ICP working
 
 standards for both EI and LC. 
 
 8.4 For anew set of standards, the following tasks must be performed: 
 
 8.4.1. Forms D-777-F1, D-777-F2, and a printed copy of the electronic form D-777-
 
 F4 should be reviewed along with a printout of the first calibration generated
 with the standards. 
 
 8.4.1.1 This review ensures that stock standard concentrations and the
 
 volumes/weights for all dilutions were transcribed correctly into D-
 
 777-F4. 
 
 8.4.1.2 This review ensures that the working standard concentrations
 generated by D-777-F4 were transcribed correctly into the ICP
 
 software. 
 
 8.4.2 After review, the documents shall be scanned into electronic format and
 archived in the F:/Laboratory/ICPMS/ folder. 
 
 8.4.3. After scanning, the printed documents will be included with the test results
 
 packet of one of the finished products analyzed during the run.
 
 
 

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 8.4.4 Thereafter, when these standards are used for an analysis, the scanned copy will
 be printed for inclusion into the test results packet of finished products tested
 
 during the run. 
 
 8.5 EI Standards Preparation 
 
 Note: The 10 ppm Mercury Solution has an expiration of 3 months if prepared in-
 
 house. The EI Intermediate Standard has an expiration of 3 months. The EI
 Working Standards have an expiration of 1 week. Keep containers closed when
 
 not in use and be sure not to mix up lids for the different concentration solutions.
 
 8.5.1 Mercury 10 ppm Solution (only required if the Stock Solution is 1000 ppm).
 
 8.5.1.1 Document preparation of the Mercury 10 ppm Solution in a
 Laboratory Notebook. 
 
 Bue lie Use a 100-mL glass volumetric flask that has been previously soaked
 
 for at least one hour in a 5% nitric acid solution and rinsed with water
 
 to remove residual contamination. 
 
 8.5.1.3 Transfer about 50 mL of water to a 100-mL glass volumetric flask.
 
 8.5.1.4 Add 10 mL of Nitric Acid. 
 
 Did Ling) Add 1.0 mL of 1000 ppm Hg Stock Solution. 
 
 8.5.1.6 Dilute to volume with water. 
 
 8.5.1.7 Close the container and mix well. 
 
 8.5.1.8 Alternatively use commercially available 10 ppm Hg standard.
 
 8.5.2. El] Intermediate Standard Preparation 
 
 8.5.2.1 Transfer 0.63 mL of Class 1 Stock, 0.63 mL of Class 2 Stock, and 6.3
 
 mL of Class 3 Stock, and 0.6 mL of 10 ppm Hg Stock to a 50-mL
 container. If not performing analysis of all elements listed in USP
 
 <232>, addition of the Class 2 and 3 Stock solutions may be omitted.
 
 
 

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 8.5.2.2 Dilute to volume with Diluent. 
 
 8.5.2.3 Close the container and mix well. 
 
 8.9.3 EI Working Standard #5 
 
 8.5.3.1 | Transfer 1.0 mL of EI Intermediate Standard to a 50-mL container.
 
 8.5.3.2 Dilute to volume with Diluent. 
 
 8.5.3.3 Close the container and mix well. 
 
 8.5.4 EI Working Standard #4 
 
 8.5.4.1 Transfer 0.75 mL of EI Intermediate Standard to a 50-mL container.
 
 8.5.4.2 Dilute to volume with Diluent. 
 
 8.5.4.3. Close the container and mix well. 
 
 8.5.5 EI Working Standard #3 
 
 8.5.5.1 | Transfer 0.5 mL of EI Intermediate Standard to a 50-mL container.
 
 8.5.5.2 Dilute to volume with Diluent. 
 
 8.5.5.3. Close the container and mix well. 
 
 8.5.6 EI Working Standard #2 
 
 8.5.6.1 | Transfer 0.25 mL of EI Intermediate Standard to a 50-mL container.
 
 8.5.6.2 Dilute to volume with Diluent. 
 
 8.5.6.3. Close the container and mix well. 
 
 8.5.7 EI Working Standard #1 
 
 8.5.7.1 ‘Transfer 0.8 mL of EI Working Standard #3 to a 50-mL container.
 
 8.5.7.2 Dilute to volume with Diluent. 
 
 8.5.7.3 Close the container and mix well. 
 
 8.6 LC Standards Preparation 
 
 
 

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 Note: The LC Intermediate Standard has an expiration of one month. The LC Working
 
 Standards have an expiration of one week. Keep containers closed when not in
 
 use and be sure not to mix up the lids for the different concentration solutions.
 Track the weight of tubes during each step of standard preparation according to
 
 D-777-F4 (LC-weight section) if quantitation is to be performed on w/w basis.
 
 Note: The presence of strontium (Sr) hinders the determination of “4Ca due to
 interference from doubly charged **Sr. Prepare strontium standards separate from all
 
 other elements. Prepare the strontium Intermediate Standard by diluting 5.0 mL of a 1000
 
 g/mL strontium stock to a final volume of 50 mL. Prepare strontium Working Standards
 in the same manner as for other LC standards. 
 
 8.6.1 LC Intermediate Standard Solution 
 
 8.6.1.1 Transfer 0.125 mL of the following 1000 g/mL single element
 
 standards to a single 50-mL container: Cr, Cu, Mn, Mo, Se, and V.
 
 8.6.1.2 Transfer 5.0 mL of the following 1000 pg/mL single element
 
 standards to the same 50-mL container: B, Ca, Fe, K, Mg, Na, P, Zn
 
 8.6.1.3. Dilute to volume with Diluent. 
 
 8.6.1.4 Close the container and mix well. 
 
 8.6.2 LC Working Standard #6 
 
 8.6.2.1 Transfer 4.0 mL of the LC Intermediate Standard Solution to a 50-
 mL container. 
 
 8.6.2.2 Dilute to volume with Diluent. 
 
 8.6.2.3. Close the container and mix well. 
 
 8.6.3 LC Working Standard #5 
 
 8.6.3.1 Transfer 3.0 mL of LC Intermediate Standard Solution to a 50-mL
 container. 
 
 8.6.3.2 Dilute to volume with Diluent. 
 
 
 

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 8.6.3.3. Close the container and mix well. 
 
 8.6.4 LC Working Standard #4 
 
 8.6.4.1 Transfer 2.0 mL of LC Intermediate Standard Solution to a 50-mL
 container. 
 
 8.6.4.2 Dilute to volume with Diluent. 
 
 8.6.4.3 Close the container and mix well. 
 
 8.6.5 LC Working Standard #3 
 
 8.6.5.1 Transfer 1.0 mL of LC Intermediate Standard Solution to a 50-mL
 
 container. 
 
 8.6.5.2 Dilute to volume with Diluent. 
 
 8.6.5.3. Close the container and mix well. 
 
 8.6.6 LC Working Standard #2 
 
 8.6.6.1 Transfer 0.4 mL of LC Intermediate Standard Solution to a 50-mL
 
 container. 
 
 8.6.6.2 Dilute to volume with Diluent. 
 
 8.6.6.3 Close the container and mix well. 
 
 8.6.7 LC Working Standard #1 
 
 8.6.7.1 Transfer 0.1 mL of LC Intermediate Standard Solution to a 50-mL
 
 container. 
 
 8.6.7.2 Dilute to volume with Diluent. 
 
 8.6.7.3 Close the container and mix well. 
 
 9.0 Sample Preparation 
 
 9.1 Sample preparation is documented on Form D-777-F3 ICP Sample Preparation Form.
 
 
 

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 9.2 Sample preparation involves either dissolution and/or dilution in Diluent (for LC samples
 where the target element is soluble and/or EI samples where the entire sample is soluble)
 
 or closed-vessel microwave digestion with subsequent dilution in Diluent (for LC
 samples where the target analyte is not soluble and/or EI samples that do not completely
 
 dissolve in the diluent). 
 
 9.3 The dilution factor and sample amount are chosen so that the final concentrations for all
 
 elements, which may correspond to either a limit or a label claim, are within the linear
 range for the analytical procedure. Multiple dilutions may be required.
 
 ea EI are always determined in units of g/mL; therefore, the dilution factor should
 
 be calculated in units of mL. 
 
 9.3.2 LC can be determined in units of either ug/g or ug/mL. 
 
 Dual Determination in units of ug/g is more precise and should generally
 
 be used for finished product release testing. This requires that the
 weight of the sample be tracked during the entire sample preparation
 
 process in order to calculate the dilution factor in units of g.
 
 ee Determination in units of ug /mL is easier but less precise and may
 be used for complex determinations such as product specific LC
 
 validations. In this case, dilution factors should be calculated in units
 
 of mL. 
 
 9.4 Finished Products 
 
 9.4.1 Test details including sample weight, dilutions, digestion reagents, and digestion
 program are included as an attachment to the finished product test ticket.
 
 9.4.1.1 The optimal sample weight and dilutions for inclusion into a Test
 
 Details page are generated using electronic form D-777-F5. In the
 case of product requiring testing of multiple label claim elements, the
 
 dilutions specified by the form may be adjusted so that fewer dilutions
 
 must be performed. However, the concentration of all target elements
 
 
 

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 in the working sample are within the linear range of the method (200
 — 8000 ng/mL for B, Ca, K, Fe, Mg, Na, P, Sr, Zn and 5 — 200 ng/mL
 
 for Cr, Cu, Mo, Mn, Se, and V). 
 
 * For example, D-777-F5 specifies a 0.4/15 mL dilution for
 
 Na and a 0.5/15 mL dilution for K. In this case, the dilution
 for K may be changed to 0.4/15 mL to reduce the total
 
 number of dilutions required. 
 
 9.4.1.2 The digestion or dissolution parameters for inclusion into a Test
 Details page shall be determined using the guidelines presented in
 
 Section 10. 
 
 9.4.2 If Test Details for a finished product are not attached to the test ticket, use
 
 electronic form D-777-F5 to determine the optimal sample preparation
 parameters including sample weight and dilutions. Instructions for using D-777-
 
 FS are included with the form. Refer to section 10 to determine digestion or
 dissolution conditions. 
 
 9.4.3. A product specific method validation is recommended in the following cases to
 
 demonstrate accuracy and precision: 
 
 9.4.3.1 Anew target element is to be analyzed for which there is no previous
 
 instance of a product specific method validation. 
 
 9.4.3.2 An aspect of the product (e.g. dosage form, sample matrix, label
 claim, elemental impurities limits, etc.) is significantly different from
 
 those which have undergone previous validation and/or is expected to
 present difficulty during sample preparation and/or analysis.
 
 9.4.3.3 If release testing has been conducted on a product that has not
 
 undergone validation and the results do not meet product release
 
 specifications, a product specific validation may be performed as part
 of an investigation conducted according to D-105. 
 
 
 

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 9.4.4 For Raw Materials: 
 
 9.4.4.1 For EI, the sample weight is always 132 mg. 
 
 9.4.4.2 For EI, the total dilution is always 50 mL. 
 
 9.4.4.3 For LC, the default sample weight is 132 mg. Other weights may be
 used. A larger sample weight may be more appropriate for a raw
 
 material with very low content of the target element and/or
 
 heterogeneous appearance. 
 
 9.4.4.4 For LC, the sample must be diluted into the linear range of the
 method. Use electronic form D-777-F6 to determine the correct
 
 dilution(s). Print the completed form D-777-F6 and include it with
 the data packet. 
 
 10.0 Sample Digestion or Dissolution 
 
 10.1. If the sample is completely soluble or miscible in the diluent (clear or colored solution,
 but not cloudy), no digestion is required. For determination of label claim elements: if
 
 the target element is completely soluble in the diluent, no digestion is required.
 
 10.1.1 Homogenize the sample thoroughly before removing a portion for testing.
 
 10.1.1.1 Solid powders of uniform particle size: mix the sample by turning
 
 end-over-end for at least 30 seconds or mix the sample by coning and
 quartering. 
 
 10.1.1.2 Solid powders of irregular particle size: grind the sample in a mortar
 
 and pestle or blender to obtain a powder of uniform particle size.
 
 10a Oe Liquid solutions or suspensions: mix the sample by turning end-over-
 end for at least 30 seconds. Sample suspensions immediately after
 
 mixing before settling occurs. 
 
 10.1.1.4 Tablets: Record the weight of at least 10 dosage units, and calculate
 
 the average tablet weight. Grind in a mortar and pestle or blender to
 
 
 

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 obtain a powder of uniform particle size. The sample weight for
 digestion should be determined as outlined above. 
 
 10.1.1.5 Capsules containing solid powder: Pool at least 10 dosage units and
 
 completely remove all fill material from the capsules. A small spatula
 may be useful in removing fill material that sticks in the capsules.
 
 Record the weight of the pooled fill material and calculate the average
 
 fill weight per capsule. Typically, the sample for digestion will be one
 capsule shell combined with an amount of powder equivalent to the
 
 average fill weight. When transferring the sample to a digestion
 vessel, record the weight of fill material and capsule shell separately.
 
 10.1.1.6 Capsules containing liquid fill: Typically, a single capsule will be
 
 digested without homogenization. 
 
 10.1.2 Place a 50-mL container directly on the balance and tare. 
 
 10.1.2.1 Other volumes may be used. 
 
 10.1.2.2 If containers are to be re-used, clean them in the following manner:
 
 ° Soak containers for at least one hour in a 1% citranox
 
 solution. 
 
 * Rinse containers with hot tap water. 
 
 ® Rinse containers with DI water. 
 
 * Allow containers to air dry before use. 
 
 10.1.3 Transfer the required sample weight to the container. 
 
 10.1.4 Dilute the sample to the final volume using Diluent. 
 
 10.1.5 Close the container and mix well. This is the Stock Sample.
 
 10.1.6 Perform any further dilutions required for EI and/or LC analysis using the
 
 dilution volumes determined in Section 9. 
 
 
 

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 10.1.7 Proceed to Section 11. 
 
 10.2 If the sample and/or target element is not soluble in the diluent:
 
 10.2.1 Homogenize the sample before removing a portion for testing as outlined in
 Section 10.1. 
 
 10.2.2 Place a digestion vessel directly on the balance and tare. 
 
 10.2.3 Transfer the sample weight calculated in Section 9 to the digestion vessel, and
 
 record the weight. 
 
 10.2.4 Transfer the digestion vessel to an exhaust hood. 
 
 10.2.5 Slowly add the required digestion reagents washing any sample that may have
 
 stuck to the sides of the vessel to the bottom during the addition. For most
 samples, 5.0 mL of Nitric Acid and 1.0 mL of Hydrochloric Acid is suitable for
 
 sample digestion. If a product specific validation is applicable, use the digestion
 reagents listed in the validation report. 
 
 10.2.6 Install the safety vent and screw cap on the vessel, but do not tighten the cap.
 
 10.2.7 Wait at least 5 minutes for pre-digestion reactions to subside. For sample sizes
 greater than 0.5 g, it is advisable to wait for at least 15 minutes with occasional
 
 swirling before proceeding. For samples containing > 10% oil, wait at least one
 hour with occasional swirling before proceeding. If a validation has been
 
 performed, use the pre-digestion time listed in the validation report. For raw
 materials, 5 minutes is sufficient. 
 
 10.2.8 Tighten the vessel screw cap until the center piece on the top is just flush with
 the outer portion of the cap. 
 
 10.2.9 Immediately place the closed vessel into the rotor body. Pressurization
 
 inside the vessel could cause irreversible bulging if the closed vessel is not
 placed into the rotor body immediately. 
 
 
 

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 10.2.10 Repeat steps 10.2.1 to 10.2.9 until all samples have been prepared for digestion.
 If fewer than 12 samples are being digested, samples should be evenly
 
 distributed (balanced) in the rotor body. When digesting multiple sample types
 in a single digestion run, it is advisable to match sample types and sample sizes
 
 as Closely as possible. 
 
 10.2.11 Place the rotor lid onto the rotor body. 
 
 10.2.12 Ensure that the drip cup and drive ring are properly installed in the microwave
 
 cavity. 
 
 10.2.13 Install the rotor into the microwave cavity and close the door.
 
 10.2.14 On the main screen, click Create Run. 
 
 10.2.15 Enter the Run Name. Ideally, the run name should uniquely identify the
 sample(s) being digested. For raw materials, the date followed by “raw mat” is
 
 sufficient. 
 
 10.2.16 Select the Method to be used for the run: 
 
 10.2.16.1 Most samples (including raw materials) 
 
 e Ramp 5 min to 75°C and hold 5 min. 
 
 e Ramp 5 min to 120°C and hold 5 min. 
 
 e Ramp 5 min to 180°C and hold 10 min. 
 
 10.2.16.2 Large sample size or reactive sample such as oi] 
 
 e Ramp 10 min to 50°C and hold 5 min. 
 
 e Ramp 10 min to 70°C and hold 5 min. 
 
 e Ramp 20 min to 180°C and hold 10 min. 
 
 10.2.17 If desired, enter a Run Note. 
 
 
 

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 10.2.18 If digesting only a single vessel, it is necessary to prepare a second “dummy”
 sample that is identical to the first. Only the first sample is analyzed. It is not
 
 required to record the sample weight of the “dummy” sample. 
 
 10.2.19 Click Start. 
 
 10.2.20 After digestion is complete, remove and open each of the vessels from the rotor
 body. After a vessel has been removed from the rotor body, it must be
 
 opened without delay or the pressure inside of the vessel could cause
 
 irreversible bulging. 
 
 10.2.2] Carefully and very slowly open the digestion vessels inside of a hood. Cover
 the top of the screw cap with a paper towel, and point the vessel away from your
 
 body while opening. 
 
 10.2.22 Transfer the digested sample, using small portions of water to ensure complete
 transfer, into a 50-mL container. 
 
 10.2.23 Slowly dilute the sample to volume (usually 50 mL) using water, close the
 
 container, and mix well. This is the Stock Sample. 
 
 10.2.24 If it was determined above that dilutions are required for EI and/or LC analysis,
 
 perform dilutions using Diluent. 
 
 11.0 Starting the Software and Instrument 
 
 11.1 Turn on the Argon gas and adjust the pressure to 80 — 90 psi. 
 
 11.2 Ensure that the Helium gas is on and adjust the pressure to 14 — 17 psi.
 11.3 Ensure that there is sufficient rinse solution to complete the analysis.
 
 11.4 Ensure that there is sufficient Internal Standard Solution to complete the analysis.
 
 es Place the internal standard line into a container of Water. 
 11.6 Ensure that the waste container is not full. 
 
 11.7 Ensure that the 5% Nitric Acid rinse is in position 1 and the bottle is open.
 
 11.8 Ensure that a container of DI water is in position 2 and the bottle is open.
 
 
 

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 11.9 Ensure that the Tuning Solution containing 1 ppb each of Ce, Co, Li, Tl, and Y is in
 position 3 and the bottle is open. 
 11.10 Ensure that the Diluent solution is in position 4 and the bottle is open.
 
 Ebel Stretch the peristaltic pump tubing across the guides on the sampler and autosampler
 
 pumps. 
 ee Turn on the recirculating chiller. 
 
 11.13 Click Start—ICP-MS MassHunter Workstation—ICP-MS Instrument Control.
 11.14 Enter User ID and Password. 
 
 11.15 In the dialog box, select Instrument Control. 
 
 11.16 Double click the Plasma gadget on the Dashboard. 
 Lie In the dialog box, ensure that “execute configured ignition sequence” is selected and click
 
 OK. 
 11.18 After several minutes, the plasma is ignited. 
 
 11.19 If necessary, adjust the peristaltic pump tubing tension by reducing the tension until no
 flow is observed, slowly increasing tension until flow just starts, and then increasing
 tension | '4 additional turns. 
 
 11.20 On the Menu bar, select View—Instrument Status. If no Menu bar is shown, press the
 F10 key. 
 
 ieer Ensure that the Analyzer Pressure is 1x10° —5x10*. If the Analyzer Pressure is not within
 the required range, maintenance of the cones or vacuum system may be required.
 
 11.22 After instrument warmup, the instrument will perform startup tuning.
 
 L123 Creating a Batch 
 
 11.23.1 Select Open Batch From Template from the Batch gadget pull-down menu.
 
 11.23.2 Select the appropriate template depending on the current analysis type (EI or
 LC), and click OK. 
 
 11.23.3. In the Save Batch As dialog box, enter either EI or LC without any spaces for
 
 the type of analysis being performed. The software will append the date and time
 to create a unique batch name. 
 
 11.23.4 The software will create the new Batch. 
 
 
 

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 11.23.5 In the Batch pane, click on the Sample List tab. 
 
 11.23.6 Under Acquisition Order, select Unknown Samples. 
 
 11.23.7 Enter Sample for Sample Type, Sample Name, and Vial # for the samples. Do
 not enter File Name since the instrument will automatically generate the File
 
 Name. 
 
 11.23.8 Under Comment, enter the units for the samples. The units should match the
 
 specification listed on the test ticket. 
 
 11.23.9 Enter the Sample Weight in g for each sample. For capsules: use the weight of
 fill material only for LC, and use the weight of fill material plus capsule shell
 
 for EI. 
 
 11.23.10 Enter the Final Volume or Final Weight for each sample (FV). This 1s calculated
 by taking into account the volume or weight of the initial stock solution in
 
 addition to all subsequent dilutions. 
 
 11.23.10.1 Ifthe determination is to be made in units of ug/mL, calculate all
 
 dilutions in units of mL. For example, if the stock solution volume
 is 50 mL and a 0.4 mL aliquot of the stock solution was diluted to
 
 15 mL, the Final Volume (FV) 1s: 
 
 15mL 
 FV = 50mL x = 1875mL 
 0.4mL 
 11.23.10.2 Ifthe determination is to be made in units of ug/g, track the weight
 
 of the sample at each step in the sample preparation process and
 calculate all dilutions in units of g. Electronic form D-777-F7
 
 should be used to calculate the dilution factors. For example, if the
 stock solution weight is 50 g and a 0.4 g aliquot of the stock
 
 solution was diluted to 15 g, the Final Weight (FV) is:
 
 FV =50q xo = 1875 
 is 04g — g 
 
 
 

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 11.23.11 Enter the Dilution Multiplier (DM) for each sample. Calculation of the DM will
 depend on sample type and specification: 
 
 11.23.11.1 Elemental Impurities 
 
 e If the specification is in ug/g (ppm), enter 0.001 for DM.
 This includes most raw material samples. 
 
 e If the specification is in ug/day, then DM is the maximum
 daily dose (MDD) in units of kg/day. This typically only
 
 applies to finished products. The MDD is calculated by
 multiplying the maximum recommended number of servings
 
 per day (from the product label) by the weight of a single
 serving (from the product profile or the weight variation
 
 test). For example, if the product directions instruct to take
 
 two capsules per day and each capsule weighs 0.5 g, then the
 MDD is 2 caps/day x 500 mg x 0.000001 kg/mg = 0.001
 
 kg/day. 
 
 11.23.11.2 Label Claim 
 
 * If the specification is in %, then DM is 0.0000001.
 
 ® If the specification is in ug, then DM 1s the weight of a single
 
 dose or serving (formula weight, FW) in g divided by 1000.
 This typically only applies to finished products. FW is the
 
 average weight of at least 10 dosage units (fill material only
 
 for capsules). If testing a powder or liquid, use the FW from
 the Product Profile. 
 
 e If the specification is in mg, then DM is the FW in g divided
 
 by 1,000,000. 
 
 11.23.12 If the plasma will need to be shut down automatically at the end of the run
 (overnight run), delete the pauses at the end of the unknown samples.
 
 
 

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 11.23.13 Click Validate Method. If any errors are displayed in the Method Error List
 pane, double-click the error message to view the line in the Method Table
 
 pane that contains the error. Fix the problem and click Validate Method again.
 
 11.23.14 Place the samples in the Autosampler. The blank (Diluent) is always in
 
 position 3101, and standards are always in positions 3102 — 3106 (low to high
 conc) for EI or positions 3102 — 3107 (low to high conc) for LC.
 
 I Click Add to Queue. The acquisition starts automatically if no other task is
 
 currently running. 
 
 11.23.16 The instrument will perform tuning before running the samples.
 
 11.23.17 After tuning, the queue will pause and a performance report will be printed.
 Review the Performance Report, and ensure that the following tuning
 
 requirements are satisfied: 
 
 11.23.17.1 Mass 59 Count > 1000 
 
 11.23.17.2 Mass 89 Count > 500 
 
 11.23.17.3. Mass 205 Count > 2500 
 
 11.23.17.4 Oxide NMT 0.35% 
 
 11.23.18 If the system meets the tuning requirements, proceed to step 11.2.22.
 
 11.23.19 If the system does not meet the tuning requirements, evaluate the root cause
 
 of tune failure and repeat the auto-tune: 
 
 11.23.19.1 Tuning failures are frequently caused by worn tubing or
 
 improper tension adjustment of the peristaltic pump.
 
 e Replace tubing. 
 
 e Adjust tension on the peristaltic pump so that there is a
 smooth flow though the tubing. 
 
 11.23.19.2 Tuning failures are frequently caused by worn or dirty cones.
 
 
 

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 Remove and inspect the sampler and skimmer cones. Take
 
 great care not to damage the tip of the cones. 
 
 To clean dirty cones: sonicate for 5 min in a 1% aqueous
 citranox solution, sonicate for 5 min in deionized water, then
 
 rinse thoroughly with deionized water and dry using a gentle
 stream of argon gas. 
 
 If the orifice of the sampler cone is > 1.1 mm or the orifice
 of the skimmer cone is > 0.42 mm, replace the cone.
 
 11.23.19.3 Repeat the autotune: 
 
 Click the Batch gadget. 
 
 Click the Autosampler tab, and double click position 3.
 
 Wait 3 minutes for the tuning solution to equilibrate.
 
 Click Start Editing Mode. 
 
 Click the Start Auto Tune tab. 
 
 The instrument will perform an auto-tune and generate a
 performance report. 
 
 Click End Editing Mode. 
 
 11.23.19.4 If the system meets the tuning requirements, proceed to step
 11.2.20. 
 
 11.23.19.5 Ifthe system still does not meet the tuning requirements, contact
 
 Perkin Elmer customer support. 
 
 11.23.20 Place the internal standard line in the internal standard solution. Wait for five
 minutes, then click the Queue drop down menu and select Resume.
 
 11.23.21 The instrument will measure the 5% nitric acid solution. After measurement
 
 is complete, the queue will be paused. 
 
 
 

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 11.23.22 Right-click on the Queue drop down menu and select Resume.
 
 11.23.23. The calibration will be performed. After the calibration is complete, the queue
 will pause. Check the calibration for each target element in online data
 
 analysis: 
 
 11.23.23.1 Click on any column under the target element on any standard
 
 row. The calibration curve and table will be displayed in the
 bottom right pane. 
 
 11.23.23.2 In the calibration table, ensure that the concentration and
 
 measured concentration are in good agreement for all
 concentration levels. 
 
 11.23.23.3. Ensure that R 1s: 
 
 e EI: Ris NLT 0.995 
 
 e LC: Ris NLT 0.997 
 
 11.23.23.4 Ensure that the background equivalent concentration (BEC) for
 the elements to be quantified conform with the limits listed in
 
 Table 1: 
 
 Table 1: BEC Limits 
 
 Element BEC Limit 
 As, Cd, Hg, Pb 0.03 
 B, Ca, Fe, K, Mg, Na, P, Zn 100 
 Cr, Cu, Mn, Mo, Se, V 2 
 
 11.23.23.5 Ifthe calibration does not meet the requirements above, abort the
 
 run and prepare new standards and/or Diluent. 
 
 11.23.23.6 If the calibration meets the requirements, right-click on the
 
 Queue drop down menu and select resume. 
 
 11.23.24 The instrument will now run the samples. 
 
 
 

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 11.23.25 To check the progress of the acquisition, click the Queue gadget on the
 
 Dashboard. The progress is displayed in the status bar in the lower right
 
 corner. 
 
 11.23.26 To modify a batch that is currently running in the queue:
 
 11.23.26.1 Click the Batch gadget. 
 
 11.23.26.2 Inthe Batch pane, click on the Sample List tab. 
 
 11.23.26.3 Under Acquisition Order, select Unknown Samples.
 
 11.23.26.4 Select the line just below the currently running sample.
 
 11.23.26.5 Click Start Editing Mode on the toolbar in the Batch pane.
 
 11.23.26.6 When all changes have been made, click End Editing Mode.
 
 11.23.27 To automatically turn off the plasma when all queue items have been
 
 completed, select Plasma Off at the End on the toolbar in the Queue pane. All
 pauses at the end of the unknown samples must be removed, or the queue will
 
 not reach the plasma off directive. Note: The preferred method of instrument
 shut down is outlined in Section 11.5; however, using Plasma Off at the End
 
 is acceptable for overnight runs. 
 
 11.24 Generating the Report 
 
 11.24.1 The procedure for generating a report is the same in both Online and Offline
 
 Data Analysis. 
 
 11.24.2 Click the Report gadget on the ICP-MS Data Analysis window.
 
 11.24.3 Select the samples for which a report is needed. 
 
 11.24.4 In the Generate Report dialog box, select the Ion Labs Report Template,
 Selected Samples, and To Printer. 
 
 11.24.5 Click Generate 
 
 
 

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 11.24.6 Click the drop down menu of the Report Gadget, and select Print Calib
 Simple. 
 
 11.24.7. Click the drop down menu of the Report Gadget, and select Show Tune
 
 Report. When the Tune Report is displayed, right click on the report and select
 Print. 
 
 11.25 Reporting Requirements 
 
 11.25.1 Sample Report. 
 
 11.25.2. D-777-F1 Intermediate Standard Preparation Form 
 
 11.25.3. D-777-F2 Working Standard Preparation Form 
 
 11.25.4 D-777-F3 Sample Preparation Form (finished products only).
 
 11.25.5 D-777-F4 Standard Concentration Calculations 
 
 11.25.6 D-777-F6 Raw Material Sample Preparation Calculations (only LC)
 
 11.25.7. D-777-F7 Dilution by Weight Calculations (only for LC if dilutions
 performed by weight). 
 
 11.25.8 Lab notebook containing sample weights and dilutions (raw materials only).
 
 11.25.9 Tuning Report. 
 
 11.25.10 Calibration Report. 
 
 11.25.11 D-777-F8, ICP Data Review Checklist 
 
 11.26 Electronic Data and Audit Trail Review 
 
 11.26.11 All results generated shall have the electronic data reviewed and compared to
 result reports. Review should include the following: 
 
 11.26.1.1 Standard Concentrations 
 
 11.26.1.2 Sample weights, multiplier, and dilutions 
 
 11.26.2 Audit trail shall be reviewed for traceability and completeness.
 
 
 

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 11.26.2.1 Testing present should align with documentation during audit trail
 review. 
 
 11.26.2.2 All creation and modification actions captured in the audit trail
 
 should align with normal practices. 
 
 11.27 Instrument Shutdown 
 
 11.27.1 Double click the Plasma gadget on the dashboard. 
 
 11.27.2 In the dialog box, click Yes. 
 
 LL.27:3 Click the Batch gadget. Select the Autosampler tab, and double click Home.
 
 11.27.4 Close the Instrument Control window. If Instrument Control is not
 
 closed, other users will not be able to use the instrument.
 
 11.27.5 Close all sample and standard containers. 
 
 Lig/.6 Release tension on the peristaltic pump tubing. 
 
 11.28 Digestion Vessel Cleaning 
 
 11.28.1 Soak vessels for at least one hour in a 1% citranox solution.
 
 11.28.2 Rinse vessels with hot tap water. Be sure to pass water through the pressure
 release valve. 
 
 11.28.3 For difficult to remove contamination: add 4 mL of water and 3 mL of nitric
 acid to the digestion vessel. Perform a digestion cycle ramping the
 
 temperature to 180°C over 10 minutes and hold at 180°C for 10 minutes.
 
 11.28.4 In some cases, a rinse with organic solvent (methanol or isopropanol) may be
 required to remove partially digested samples. 
 
 11.28.5 Only for extremely resistant residual contamination, a test tube brush may be
 
 used. Take extreme care not to scratch the Teflon vessel with the test tube
 brush. 
 
 
 

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 11.28.6 Rinse vessels with DI water. Be sure to pass water through the pressure release
 valve. 
 
 11.28.7 Allow vessel to air dry before use. 
 
 12.0 Acquisition Method 
 
 12.1. Parameters Applicable to EI and LC 
 
 12.1.1 Peri-Pump Parameters 
 
 12.1.1.1 Uptake Speed 0.5 rps 
 
 12.1.1.2 Uptake Time 45 sec 
 
 12.1.1.3 Stabilize Time 30 sec 
 
 12.1.1.4 Probe Rinse Speed 0.5 rps 
 
 12.1.1.5 Probe Rinse Time Spl 20 sec 
 
 12.1.1.6 Probe Rinse Time Std 20 sec 
 
 12.1.1.7 Rinse Vial 1 Speed 0.5 rps 
 
 12.1.1.8 Rinse Vial 1 Time 60 sec 
 
 12.1.1.9 Rinse Vial 4 Speed 0.5 rps 
 
 12.1.1.10 Rinse Vial 4 Time 60 sec 
 
 12.1.1.11 Intelligent Rinse Off 
 
 12.1.1.12 Preemptive Rinse On 
 
 12.1.1.13 Start Rinse Before Acq Complete 30 sec 
 
 12.1.2 Internal Standards 
 
 Table 2: Internal Standard Masses Monitored 
 
 Element Monitored Mass Integ Time 
 Se 45 1.5 
 Ge ip: 1.5 
 
 
 

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 b 89 0.3 
 Bi 209 0.3 
 12.2 EI Specific Parameters 
 
 12.2.1 Acq Parameters 
 
 12.2.1.1 Acq Mode Spectrum 
 
 12.2.1.2 Peak Pattern 3 Point 
 
 12.2.1.3. Replicates 3 
 
 12.2.1.4 Sweeps/Replicate 100 
 
 12.2.2 Tune Parameters 
 
 12.2.2.1 Use Gas Yes 
 
 12.2.2.2. He Flow On 
 
 12.2.2.3 He Flow Rate 4.8 
 
 Table 3: Target Element Masses Monitored for Elemental Impurities 
 
 Monitored Internal Monitored Internal . 
 Element Mass Std Integ Time | Element Wass Std Integ Time 
 Li 7 45 6.0 Ag 107 89 0.5 
 V 5] 45 0.5 Cd 111 89 1.0 
 Cr ae 45 0.5 Sn 118 159 0.3 
 Co 59 45 0.3 Sb 121] 159 0.3 
 Ni 60 45 0.5 Ba 137 159 0.3 
 Cu 63 72 0.3 Os 189 159 0.5 
 AS 75 72 3.0 Ir 193 209 0.5 
 Se 78 72 3.0 Pt 195 209 0.5 
 Mo 95 89 0.3 Au 197 209 0.5 
 
 Ru 101] 89 0.3 Hg 202 209 2.0 
 Rh 103 89 0.3 af 205 209 0.3 
 Pd 105 89 0.3 Pb 208 209 0.3 
 
 12.3. Label Claim 
 
 12.3.1 Acq Parameters 
 
 
 

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 12.3.1.1 Acq Mode Spectrum 
 
 12.3.1.2 Peak Pattern 3 Point 
 
 12.3.1.3. Replicates + 
 
 12.3.1.4 Sweeps/Replicate 100 
 
 12.3.2 Tune Parameters 
 
 12.3.2.1 Use Gas Yes 
 
 12.3.2.2 He Flow On 
 
 12.3.2.3. He Flow Rate 4.8 
 
 Table 4: Target Element Masses Monitored for Elemental Impurities 
 
 EI ‘ Monitored Internal Integ Blenent Monitored Internal Integ 
 —— Mass Std Time Mass Std Time 
 B 1] 45 2.0 Cr 52 45 0.5 
 Na 23 45 0.3 Mn 55 45 0.5 
 Meg 24 45 0.5 Fe 56 45 0.5 
 P 31 45 0.3 Cu 63 45 0.5 
 K 39 45 0.3 Zn 66 72 2.0 
 Ca 44 45 2.0 Se 78 72 2.0 
 V 5] — 45 | 0.5 Sr 88 89 2.0 
 | De Mo 95 89 0.5 
 
 13.0 ICP-MS Maintenance 
 
 13.1 Routine maintenance of the ICP-MS is documented in a laboratory notebook.
 
 13.2 Perform maintenance as outlined in the Hardware Maintenance Manual or in the
 
 instructional videos located at D:/ICPMS Resources/Maintenance videos/index. html.
 
 13.3 Recommended maintenance frequency: 
 
 13.3.1 Tubing Replace weekly 
 
 13.3.2 Nebulizer Clean if blocked or if memory effects are observed
 
 13.3.3. Spray Chamber Clean 1f memory effects are observed 
 
 
 

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 13.3.4 Torch Clean if deposits are observed, replace if damaged
 
 13.3.5 Torch Shield Plate Replace if corroded or deformed 
 
 13.3.6 Torch Bonnet Replace if fractured, cracked, or chipped 
 
 13.3.7 RF Coil Replace if corrosion or damage is observed 
 
 13.3.8 Sampling Cone Replace if orifice is > 1.10 mm, deformed, or damaged
 
 13.3.9 Skimmer Cone Replace if orifice is > 0.42 mm, deformed, or damaged
 
 13.3.10 Graphite Gasket Replace if pitted, damaged, or worn 
 
 13.3.11 Ion Lens Clean every three months 
 
 13.3.12 Vacuum Pump Change pump oil every six months, check level monthly
 
 13.3.13 Cooling Fluid Replace yearly, check level monthly 
 
 14.0 Consumables List 
 
 14.1 Sample Tubing White/White SCP Science P# 022-133-009 
 
 14.2 Internal Standard Tubing Orange/Blue Agilent P# 5005-0021 
 
 14.3 Waste Tubing Santoprene Yellow/Blue SCP Science P# 022-033-419 
 
 14.4 MicroMist nebulizer Agilent P#G3266-80004 SCP Science P# 020-060-047
 
 14.5 Torch, quartz, 2.5 mm 1.d. Agilent P# G3280-80053 SCP Science P# 020-056-005
 
 14.6 Shield plate Agilent P# G1833-65419 SCP Science P# 020-102-156 
 
 14.7 Bonnet Agilent P# G1833-65421 SCP Science P# 020-056-052 
 
 14.8 Nickel Sampling Cone Agilent P# G3280-67040 SCP Science P# 020-102-073
 
 14.9 Nickel Skimmer Cone Agilent P# G3280-67041 SCP Science P# 020-102-074
 
 14.10 Graphite Gasket for Sampling Cone Agilent P# G3280-67009 
 
 14.11 Extraction-Omega Lens Assembly Agilent P# G8400-67001 
 
 14.12 Vacuum Pump Oil Agilent P# X3760-64004 
 
 
 

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 14.13 Oil mist filter element Agilent P# G1960-80039 
 
 15.0 Product Specific Method Validation 
 
 15.1 Product specific method validation is not required for finished products; however,
 
 validation is recommended in certain cases (see Section 9.4). 
 
 15.2 Validation will generally not be performed for raw materials. 
 
 15.3 The method validation requirements outlined herein are applicable to the majority of
 finished products at ION Labs. If a material has specific validation requirements due to
 
 its intended use, customer request, or any other reason, a separate validation protocol may
 be created for the product. 
 
 15.4 Product specific validations should not be performed concurrently with finished product
 
 or raw material release testing. Acquire validation results in a separate batch.
 
 15.5 Do not use the finished product batch number as a sample name in validation batches.
 
 Instead, use the product formula number. 
 
 15.6 Validation requirements differ by use class as indicated below. 
 
 15.7 System Suitability: 
 
 15.7.1. The tuning requirements outlined in Section 11.2.17 are met.
 
 15.7.2 The calibration requirements outlined in Section 11.2.23 are met.
 
 15.8 Specificity: Detection by mass spectrometry is inherently specific. However, certain
 
 elements are subject to interferences from polyatomic species generated by other
 elements, matrix components, and plasma gasses as well as isobaric interferences from
 
 other elements which have isotopes at the same mass as the target element. As suggested
 in USP <233>, the use of a collision cell purged with an appropriate gas will be utilized
 
 to reduce polyatomic interferences. Isobaric interferences are mitigated by carful choice
 of the monitored isotope for each target element. Specificity is demonstrated by the
 
 assessment of accuracy as outlined below. 
 
 15.9 Precision (Repeatability): 
 
 
 

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 13.9.1 Prepare replicate samples, and analyze them by the ICP-MS method. Calculate
 the %RSD for the measured concentrations of the replicate samples. Solid and
 
 liquid suspensions requiring digestion must be spiked prior to digestion if
 digestion is required. 
 
 15.9.2 The number of replicate samples depends on the use class of the material as
 
 follows: 
 
 15.9.2.1 Drug: Six replicate samples. 
 
 15.9.2.2 Dietary Supplement, Cosmetic, and Food: Three replicate samples.
 
 15.9.2.3 If a material fits into multiple use classes, perform validation based
 
 on the most restrictive class. 
 
 [3.9.3 The spike level and acceptance criteria depend on the analyte class as follows:
 
 15.9.3.1 Elemental Impurities: Spiked at the limit level (1.00J). The %RSD is
 NMT 20%. 
 
 15.9.3.2 Label Claim Elements: Not spiked. In general, the %RSD should be
 
 NMT 10%. For label claim elements which are present at very low
 
 levels in the finished product (< 0.1%), an acceptance criteria of NMT
 20% RSD is more appropriate. 
 
 15.10 Accuracy: 
 
 15.10.1 Prepare replicate samples spiked with the target elements and analyze them by
 
 the analytical procedure. Solid and liquid suspensions requiring digestion must
 be spiked prior to digestion if digestion is required. Evaluate the recovery of
 
 spiked elements. 
 
 15.10.2 The spike level and number of replicate samples depends on the use class and
 
 analyte type as follows: 
 
 15.10.2.1 Elemental Impurities 
 
 
 

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 e Drug: Three replicate samples spiked at 0.5J, six replicate
 samples spiked at 1.0J, and three replicate samples spiked at
 
 1.5J. Additionally, prepare triplicate unspiked samples as a
 
 control. 
 
 e Dietary Supplement, Cosmetic, and Food: Three replicate
 samples spiked at 1.0J. Additionally, prepare three replicate
 
 unspiked samples as a control. 
 
 e Ifa material fits into multiple use classes, perform validation
 
 based on the most restrictive class. 
 
 15.10.2.2 Macro-Elements 
 
 e All use classes: Three replicate samples spiked at one half of the
 label claim amount. Additionally, prepare three replicate
 
 unspiked samples as a control. 
 
 15.10.3 The acceptance criteria depends on the analyte class as follows:
 
 15.10.3.1 Elemental Impurities: The average (mean) recovery of spiked
 
 elemental impurities in the replicate samples is 70% - 150%:
 
 15.10.3.2 Label claim elements: In general, the average (mean) recovery of
 spiked label claim elements should be 90% - 110%. For elements
 
 which are present at very low levels in the finished product (< 0.1%),
 an acceppt ance criteria of 80% - 120% recovery y is more apppprrooppriate.
 
 15.11 Linearity/Range: Use the System Suitability requirements for validation of linearity.
 
 15.12 Quantitation Limit: As discussed in USP <233>, sufficient sensitivity of the method
 
 (Quantitation Limit) is demonstrated by meeting the requirements for Accuracy.
 
 16.0 Quality Assurance Activities 
 
 16.1 Each QC Laboratory analyst that performs testing by ICP-MS is required to participate
 
 in a quality assurance activity at least once annually. 
 
 
 

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 16.2 Quality assurance activities which may satisfy this requirement include proficiency
 
 testing, inter-laboratory comparison, and intra-laboratory comparison.
 
 16.3 Requirements for quality assurance activities are outlined in SOP D-118 Laboratory
 
 Quality Assurance Activities Plan (Proficiency Testing). 
 
 17.0 Documentation Requirements 
 
 17.1 All documentation will be completed as outlined in SOP A-106 Documentation
 Guidelines for cGMP Records. 
 
 Li.2 All documentation will be distributed and maintained as outlined in SOP C-501
 Document Control and SOP C-502 Record Storage, Retention, and Destruction.
 
 18.0 Revision History 

| Rev | Date | Description of Changes | CCR # | By |
|-----|----------|------------------------|-------|----|
| 0 | 05/23/19 | New procedure. N/A S. Sassman Increased internal standard concentration. Added expiration for standard solutions, change LC standard concentration for some elements, change diluent composition. Added _ specific 09/25/19 instructions for various dosage forms. Modified procedure for 19-0607 S. Sassman more automated analysis. Added calculation and reporting instructions. Added instrument parameters. Added requirements for method validation. Added maintenance schedule. Changed internal standard prep to eliminate interferences from carbon, change LC stock standard expiry to one month. Changed standard prep to increase the concentration for Mg, allow use of 10/07/20 a blender for sample prep. Changed run name conventions for CC-20-0713 S. Sassman microwave. Changed tuning requirements. Added requirement for BEC. Updated formatting. Added prep/expiration date lines to solution prep form. Added forms for calculation of standard concentrations, calculation of validation parameters, calculation of raw material dilutions for label claim analysis, calculation of dilution factors for label claim analysis, and data review checklist. Modified 12/20/22 CC-22-0478 S. Sassman sample preparation section to point to test details section of product profile for sample weight and dilutions. Added requirements for excluding finished products from validation. Simplified validation requirements. Increased BEC limits by factor of 1000 since the unit of measure was changed from ug/g to ng/g in revision 3. Added Tb in internal 01/10/23 CC-23-0014 S. Sassman std table since it is being used, peak pattern for LC should be 3 point. | - | - |

 
 

[SOP 

 Standard Operating Procedure SOP No Rev 
 Page 
 Elemental Analysis by ICP-MS D-777 
 37 of 37 
 
 Updated to accommodate analysis of finished products for LC 
 without digestion. Updated D-777-F5 to accommodate analysis 
 of finished products for LC without digestion. Removed 
 requirement for product specific validation. Slightly increased
 5 05/19/23 CC-23-0223 S. Sassman 
 internal standard solution concentration. Added troubleshooting
 for tuning failures. Updated D-777-F6 to allow different stock
 solution volumes. Added section 16.0 Quality Assurance 
 Activities. 
 6 08/3 1/23 Added Electronic Data and Audit Trail Review Requirements CC-23-0438 J. Sassman
 Added strontium as an element, allow use of weight variation to
 7 06/03/24 CC-24-0242 S. Sassman 
 calculate dilution multiplier for elemental impurities. 
 8 03/04/25 Updated He flow rate CC-25-0053 A. Lukes